The genus mycoplasma represents a group of minute bacteria which have no cell walls. They are the smallest self-replicating prokaryotic organisms. Mycoplasma testing is an important way to control microbial contamination for biologics. At present, Chinese Pharmacopoeia recommends that both agar and broth media procedure and indicator cell culture procedure (DNA staining method) should be used for mycoplasma examination in master cell bank, working cell bank, virus seed batch, control cell and clinical cells. Agar and broth media procedure should be used to detect mycoplasma in the viral harvest fluid and stock fluid of viral vaccines. When necessary, culture medium can also be selected by indicated cell culture method. Other methods approved by the national drug inspection institute can also be used.